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Your DNA Repairman Is Handy as an Octopus

first_imgSome 10 times a day in a given cell, your DNA breaks on both strands.  This is an emergency.  Unless repaired quickly, serious diseases, like cancer, can develop.  But no fear: the first responder is an octopus-shaped protein complex that rushes to the rescue, wraps around the damaged site, and brings in all the parts needed to fix it.  Such mechanical acrobatics in the cell are only now coming into clearer focus.    A press release at the Scripps Research Institute described this amazing repair system called MRN with its three protein subunits (Mre11-Rad50-Nbs1; for illustration, see Science Daily).  The researchers wanted to find out how MRN “can repair DNA in a number of different, and tricky, ways that seem impossible for ‘standard issue’ proteins to do,” the press release said.  These proteins are not static balls of amino acids; they have dynamic, interactive, moving parts.  The motor in the complex, Rad50, “is a surprisingly flexible protein that can change shape and even rotate depending on the task at hand.”  Here’s the octopus part of the story:The scientists say that the parts of the complex, when imagined together as a whole unit, resemble an octopus: the head consists of the repair machinery (the Rad50 motor and the Mre11 protein, which is an enzyme that can break bonds between nucleic acids) and the octopus arms are made up of Nbs1 which can grab the molecules needed to help the machinery mend the strands. They saw “a lot of big movement” in the repair operation.  First, the complex has to assess the damage:When MRN senses a break, it activates an alarm telling the cell to shut down division until repairs are made.  Then, it binds to ATP (an energy source) and repairs DNA in three different ways, depending on whether two ends of strands need to be joined together or if DNA sequences need to be replicated.  “The same complex has to decide the extent of damage and be able to do multiple things,” [John] Tainer [Scripps Research Professor] said.  “The mystery was how it can do it all.”Tainer described how some of the parts interact: “Rad50 is like a rope that can pull.  It appears to be a dynamic system of communicating with other molecules,” he said.  It uses ATP, the energy currency of all life, to get into shape: “When not bound to ATP, Rad50 is flexible and floppy, but bound to ATP, Rad50 snaps into a ring that presumably closes around DNA in order to repair it.”    How a set of proteins can sense damage, migrate to a repair site, assess the extent of the break and select the correct repair option, link up to other tools, bring in parts, and put everything back together again is surely one of the wonders of biology coming to light with new observing techniques.  The research was funded by the National Cancer Institute, the National Institutes of Health, and the Department of Energy, and published in Nature Structural and Molecular Biology,1 March 27, 2011.    The abstract did not mention evolution except to say that the parts are “conserved” (unevolved) across all living things.  The researchers studied this complex in yeast and archaea – among the simplest of microbes.  A different paper in a different journal studied another wonder of the cell without mentioning evolution (except to mention “evolutionarily conserved proteins”).  In PLoS Biology,2 Linton Traub [U of Pittsburgh] discussed how proteins coat vesicles that dive into the cell membrane to bring in substances from outside the cell.    In “Regarding the Amazing Choreography of Clathrin Coats,” Traub described clathrin-mediated endocytosis (see 10/17/2003, 05/15/2005, 11/04/2005, 02/02/2010, bullet 3).  He started with a recounting of the discovery of clathrin, a three-spoke protein that wraps around vesicles like a geodesic dome, and then described some of the latest findings: “Yet, what we have learned over the past decade is that the assembly of these core components is augmented and precisely regulated at vesicle bud sites by an abundance of additional proteins” – at least 40, at last count.  The realization that so many players are involved in this critical import process “puts to rest the parsimonious assertion that the complexity of clathrin coat assembly is wildly overstated,” he said.1.  Williams…Tainer et al, “ABC ATPase signature helices in Rad50 link nucleotide state to Mre11 interface for DNA repair,” Nature Structural and Molecular Biology, (published online 27 March 2011), doi:10.1038/nsmb.2038.2.  Linton M. Traub, “Regarding the Amazing Choreography of Clathrin Coats,” Public Library of Science: Biology (PLoS Biol) 9(3): e1001037. doi:10.1371/journal.pbio.1001037>The facts themselves scream intelligent design so clearly, any additional comments would be superfluous.  Was evolution useful to any of this research?  Does an octopus need a hot air balloon?(Visited 36 times, 1 visits today)FacebookTwitterPinterestSave分享0last_img read more

LEBOR put on pause

first_imgShare Facebook Twitter Google + LinkedIn Pinterest U.S. District Judge Jack Zouhary pushed pause on the enforcement of the Lake Erie Bill of Rights (LEBOR) passed by Toledo voters in February.On March 18, Judge Zouhary  issued a preliminary injunction on LEBOR in the lawsuit filed by Wood County farmer Mark Drewes after Toledo voters passed LEBOR in a special election. Both Toledo and Drewes agreed to the injunction, which is a positive step according to Ohio Farm Bureau.“Farm Bureau stands strong with Mark and his family and we appreciate that this injunction will prevent the law from taking effect while the case filed by the Drewes family is litigated,” said Adam Sharp, executive vice president of the Ohio Farm Bureau. “We are happy to see the Court order a preliminary injunction delaying the enforcement of the Lake Erie Bill of Rights. This decision is one step closer to protecting farmers in the Lake Erie Watershed from costly lawsuits brought on by LEBOR.”last_img read more

Former HP CM Virbhadra Singh, wife move HC challenging trial court order in DA case

first_imgFormer Himachal Pradesh Chief Minister Virbhadra Singh and his wife Pratibha Singh on Wednesday moved the Delhi High Court challenging a trial court order to frame charges against them for allegedly amassing disproportionate assets (DA) of over ₹10 crore. The 82-year-old Congress leader and his wife have sought quashing of the December 10, 2018 trial court order directing framing of charges against Singh, his wife and seven others in the case lodged by CBI. The trial court is yet to formally frame the charges against the couple and the matter is listed for January 29. The court had in its December order said that Singh intended to cause loss to tax authorities by presenting unaccounted money as sale proceeds of apples and had also ordered framing of charges against his wife and seven others for allegedly abetting in the offences. The CBI had registered the case against Singh and others for allegedly amassing assets worth around ₹10 crore disproportionate to their known source of income when he was the Union Minister in the UPA government.last_img read more

Jermain Defoe praises A.F.C. Bournemouth ahead of expected transfer

first_imgSunderland F.C. striker Jermain Defoe has praised A.F.C. Bournemouth ahead of his expected move to the Premier League club.Defoe, who scored 15 goals in 37 league appearances for relegated Sunderland F.C., had a medical at A.F.C. Bournemouth on Tuesday and is expected to re-join the south coast club where he had a year-long loan spell in 2000-01.”What I want to do is… focus on England, the two games, and then after that everyone will know,” Defoe told reporters ahead of Saturday’s World Cup qualifier against Scotland.”It’s documented about the Bournemouth thing. It’s a club close to my heart anyway, for obvious reasons…”It’s a special place, with a top manager, and a team that’s done so well from where they’ve come from. A unique story.”Defoe urged Wayne Rooney to look for regular playing time even if it was away from Old Trafford after England’s record goalscorer was left out of Gareth Southgate’s squad for the World Cup qualifier away to Scotland and a friendly with France.The 31-year-old forward, who has lost his place in Manchester United F.C.’s first team, was also left out by England earlier this year for matches against Germany and Lithuania.”I love Wazza. He’s an unbelievable player. Someone who’s done so much,” Defoe said.”I read things about him and sometimes I’m quite taken aback… He came into the game young and played a lot of football. It’s not easy on the body, and mentally with all that pressure he’s under – probably more than most of the lads.advertisement”I think he knows he’s got to play regular football. All I’d say is I wish him the best. He’s a top player and the lads love him here. Hopefully he’ll get the chance to go somewhere and express himself, and just enjoy playing.”England travel to Scotland on June 10 ahead of a trip to France on June 13.last_img read more

Singapore Detains Bulker Long Bright

first_imgzoom The 37,300 dwt bulk carrier Long Bright has been detained in Singapore waters, according to data provided by the Supreme Court of Singapore.The 2012-built vessel, operated by China’s Highrich Logistics, was arrested in the afternoon hours of March 28, 2018.Focal Investigation & Security Agency has been appointed for security and investigation purposes related to the arrest of the vessel which was built at China’s Shandong Huahai shipyard.The bulker, which features a length of 190 meters and a width of 28.5 meters, is currently located at a PaxOcean dock in Tuas, Singapore.VesselsValue data shows that the bulker has a market value of USD 11.2 million.World Maritime News Stafflast_img

Wilmingtons Carpenters Local 339 Donates 500 To Helmets To Hardhats Veteran Group

Wilmingtons Carpenters Local 339 Donates 500 To Helmets To Hardhats Veteran Group

first_imgWILMINGTON, MA — In recognition of Veteran’s Day, Massachusetts building trades unions, including Wilmington-based Carpenters Local 339, honored local veterans this week and raised more than $50,000 for life-changing career pathways program, Helmets to Hardhats.Helmets to Hardhats is a national nonprofit that connects National Guard, reserve and transitioning active-duty military members with meaningful, family-sustaining careers in the union building trades. Carpenters Local 339 donated $500 to the group.The Massachusetts Building Trades Council is a longtime supporter of the program, which has placed more than 575 military veterans in union construction jobs statewide.“We are so proud to support Helmets to Hardhats in providing career opportunities for our valued veterans. With these donations, we want to thank these courageous men and women for their service and send a clear message that there is a place for them with our unions,” said Frank Callahan, president of the Massachusetts Building Trades Council.The Massachusetts building trades unions represent the largest workforce in the state, and they’re focused on creating middle-class employment opportunities for families across the commonwealth, including for returning veterans. 75,000 men and women have great jobs and benefits with the union building trades, and nearly a quarter of a million children, spouses, and workers are covered by their high-quality union health plans. And through Build a Life and Building Pathways, the unions are creating new avenues of economic opportunity for women and people of color so that everyone can benefit from well-paying careers in the union construction trades.Helmets to Hardhats Executive Director Darrell Roberts recognized the generosity of the building trades unions and union contractors.“Thanks to the generous contributions of the Massachusetts building trades unions and union contractors, we’re able to ensure that our returning veterans have meaningful, well-paying careers in the union building trades. Their support has allowed us to do even more to support the commonwealth’s bravest,” said Roberts.Organizers attributed much of the partnership’s success to its focus on creating new employment opportunities, and ongoing career advancement, through extensive training. The Massachusetts building trades unions spent $48 million last year alone to ensure workers at every phase of their career had free hands-on training.(NOTE: The above press release via Carpenters Local 339.)Like Wilmington Apple on Facebook. Follow Wilmington Apple on Twitter. Follow Wilmington Apple on Instagram. Subscribe to Wilmington Apple’s daily email newsletter HERE. Got a comment, question, photo, press release, or news tip? Email wilmingtonapple@gmail.com.Share this:TwitterFacebookLike this:Like Loading… RelatedSTUDENT SPOTLIGHT: Wilmington’s Meagan Pennie Receives Scholarship From Sheet Metal Workers Local 17In “Education”WASHINGTON TO WILMINGTON: Senator Markey Says FCC Vote Imperils Community TelevisionIn “Government”Wilmington’s iPods For Wounded Veterans Travel To Arizona, Partner With Teamsters To Give Gifts To VeteransIn “Community”last_img read more

Us review Jordan Peeles horror flick holds up a dark mirror to

first_img 2019 movies to geek out over Now playing: Watch this: TV and Movies Us tells the story of Adelaide Wilson, a wife and mother whose traumatic childhood experience casts a shadow over a summer beach trip with her family. Trauma haunts survivors for years to come like a shadow they can’t shake, and Us puts the members of the Wilson family face-to-face with their own shadows: doppelgangers in red jumpsuits wielding glinting gold scissors. They’re called The Tethered, and they’ve got a score to settle with their better look-alikes.You could say Us has been hotly anticipated. It follows 2017’s Get Out, a tightly-made, critically acclaimed thriller with a social message that earned Peele an Oscar for Best Original Screenplay. It helped set him up as a bright light in the realm of thrills and suspense. In fact, he’s pretty busy these days with projects like the reboot of the Twilight Zone, for which he’s the executive producer and host, and Weird City, a YouTube Original dystopian anthology series. Lupita Nyong’o takes a good long look at herself in Us. Claudette Barius So it’s hard not to make comparisons to Get Out. But where Get Out was a tidy concept, executed in a way that deliberately and carefully guided you through the story toward the big ideas, Us is bigger, broader and perhaps less neat. One of the inevitable questions people will ask about Us is whether it’s scarier than Get Out. Peele gets the dread machine going in the first scene at a beach-side amusement park, and the tensest moments include the Wilson family trying to figure out why people outside are trying to get in. And that old horror staple, the ominous Bible verse, will never not be creepy — in this case, Jeremiah 11:11. You can see the uncomprehending terror on Lupita Nyong’o’s face as she stares at her Tethered counterpart, tears streaming down her cheeks. SXSW 2019 Jordan Peele’s Us gets a new trailer and it’s terrifying 77 Photos 2 Mar 15 • LG ‘Snow White’ makes ice cream from capsules Mar 19 • AOC, Bill Nye and the apocalypse: The insanity of SXSW 2019 1:00 SXSW 2019center_img Mar 15 • Men can now breastfeed reading • Us review: Jordan Peele’s horror flick holds up a dark mirror to Get Out See All Share your voice But while Us shoots for a next level of frightening, it runs into a few weak spots along the way.For one, we learn the Tethered aren’t just knocking on the Wilsons’ door. But that larger plot is pretty vague.The effectiveness of the movie’s humor is also tough to gauge. The audience at SXSW was extremely game, laughing uproariously, even in places where it wasn’t entirely clear whether there was a joke to be had — like when the Tethered first show up in the Wilsons’ driveway, and Gabe Wilson (Winston Duke), who’d been dadding it up hard (in the best way) since the start of the movie, goes outside with a bat and some adopted bravado to try and get these mysterious figures to leave. The audience howled.In a horror film a well-placed quip can cut the tension to not only temporarily relieve the audience but also enable the tension to build back up again. Stick it in the wrong place and the laughs just undercut everything around it.Throughout the film, we’re shown lots of mirrors and reflections, visually reflecting the theme. After all, the Wilsons are seeing themselves like they never have before, though warped.Similarly, Us mirrors Get Out. It’s more scary fare with social commentary. But the copy’s never quite the same as the original.Want another take on Us? See GameSpot’s review here. Comments Tags • It shouldn’t come as a shock that a horror film titled Us plays with the idea that perhaps we are the villains.The film’s writer, director and producer, Jordan Peele, addresses how we fear and hate those we consider to be other without examining ourselves. “Maybe the monster we need to look at has our face,” Peele told the audience at the film’s world premiere during SXSW 2019. “Maybe the evil is us.” Us opens March 22 in the US and UK. Looking in a mirror is one of the recurring motifs of Peele’s new horror flick, and the biggest metaphorical theme too. Movie reviewslast_img read more

French Oil Giant Total Loses CEO to Plane Crash

first_imgChristophe de Margerie, the chief executive officer (CEO) of French oil giant Total, died in a plane accident in Moscow on Tuesday, an official said.De Margerie and three crew members were killed when the plane they were on collided with a snow-clearing machine at Moscow’s Vnukovo International Airport, Xinhua quoted an airport spokeswoman as saying.”A plane crashed when it collided with a snow-clearing machine. Three crew members and a passenger died. I can confirm that the passenger was Total’s head de Margerie,” said spokeswoman Elena Krylova.The collision occurred during the takeoff of the Dassault Falcon business jet bound for Paris with de Margerie on board on Monday midnight Moscow time, according to the airport.Total has also confirmed the death of its CEO in the plane crash in a statement, but said the accident had left a total of five people dead.As one of the most recognisable figures among the world’s top oil executives, de Margerie, 63, was on a list of attendees at a Russian government meeting on foreign investment in Gorki near Moscow Monday.Total is the fourth largest by market value of the western world’s top oil companies behind Exxon, Royal Dutch Shell and Chevron, and also one of the top foreign investors in Russia, which accounted for about 9 percent of Total’s oil and gas output in 2013.last_img read more

Lenovo Completes Acquisition Formalities of Motorola from Google

first_imgIt’s official: Lenovo buys Motorola mobilityMotorolaMotorola has announced that the company has officially become a part of the Lenovo family.Under the deal, Lenovo will pay Google, the former owner of Motorola, a total amount of $2.91 billion dollars. The transaction will include 660 million worth cash and 750 million worth stock to be paid by Lenovo. The remaining money worth $1.5 billion will be paid to Google in three years.After the deal, Lenovo will handle the mobility device business part while Google will keep the patents portfolio.Lenovo, the prime laptop and PC manufacturer has not acquired that much of presence in the mobility segment in developed countries. On the other hand, Motorola is quite popular in those countries and is counted as one of the leading Android smartphone manufacturer. Their recent launches, Moto 360 Smartwatch, Moto G, Moto E, Moto X and Droid Turbo, are widely appreciated by the consumers. Even the latest, Nexus 6, the first smartphone featuring Android Lollipop OS is also made by Motorola.With raw Android experience, quick updates, great apps and awesome Google integration, Motorola has risen like a phoenix with good revenues and popularity.In the blog, Motorola mentioned: “We will continue to focus on pure Android experience and fast upgrades, and remain committed to developing technology to solve real consumer problems. And we will continue to develop mobile devices that bring people unprecedented choice, value and quality.”The acquisition of Motorola will add a huge amount of handset sales to Lenovo’s credit and they will hold a 8% share of the global smartphone market, which will lead them to the third best smartphone maker in the global sales, according to a report. Meanwhile, Motorola mobility alone has 5% share in the Indian smartphone market.Rick Osterloh, President and COO will still lead the operation of Motorola out of Chicago.”With an impressive portfolio of smartphones, wearables and PCs, our two companies will be uniquely positioned to push the boundaries of choice and value, and bring exciting new experiences to people everywhere,” Osterloh said.last_img read more

Cognizant plans to set up SEZ in Telangana

first_imgUS-based IT major Cognizant Technologies Services Pvt Ltd is seeking the government’s nod to build a special economic zone (SEZ) in India’s youngest state, Telangana.The company’s application to set up SEZ will be scrutinised by the Board of Approval (BoA) at its meeting on 23 February. The BoA is headed by Commerce Secretary Rita Teaotia.The proposed IT/ITeS SEZ will come up in Ranga Reddy district of Telangana and it will be spread over 2.51 hectares.Due to an introduction of minimum alternate tax (MAT) by the central government on SEZs, such projects have taken a back seat. To revive the interest in setting up SEZs, the commerce ministry has proposed the finance ministry to do away with the tax, Press Trust of India reports.At this month’s meeting, the BoA will also examine other SEZ projects, including those to be set up by Kandla Port Trust and GP Realtors. The developers of the two SEZs have asked for more time for the implementation of their projects.Kandla Port Trust — developer of multi-product SEZ at Kandla and Tuna — wants the validity period of formal approval to be extended beyond 6 May, according to the agenda note of the BoA meeting.GP Realtors Pvt Ltd has sought approval for further extension of the deadline to complete its electronic hardware and IT/ITeS SEZ in Haryana.At its previous meeting on 23 December last year, the board had given its approval for 13 SEZ developers, who had asked for more time to complete the projects.Exports from SEZs in the country reached Rs 2.21 lakh crore in the April-September period in the current fiscal year compared to Rs 4.63 lakh crore in 2014-15. About 15.44 lakh jobs were generated by these zones in the first half of 2015-16.last_img read more